| Customization: | Available |
|---|---|
| Classification: | Biochemical Reagents |
| Grade: | Br |
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HCM013 Tetrathionate Broth Base (Muller-Kauffman Tetrathionate Broth)
Usages: For selective enrichment of Salmonella spp.
Principle:
Peptone and beef extract powder provides carbon, nitrogen and vitamins to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; calcium carbonate can neutralize bacterial acid production and absorption of toxic metabolites; bile and brilliant green E. coli and other groups can inhibit Gram-positive bacteria.
Formulation (per liter):
| Peptone 9g |
| Beef extract powder 4.5g |
| Sodium chloride 2.7g |
| Calcium carbonate 40.5g |
| Anhydrous sodium thiosulfate 31.9g |
| Ox bile 5g |
| Final pH7.0 ± 0.2 |
How to use:
1.Suspend 93.6g in 1L of distilled or deionized water. stirring heated to boiling until completely dissolved, packed in flasks.
2.Place the flask into sterile pot, autoclave at 121 for 20min, cooled to 30 , adding supplement.
3.Diluted and treated samples.
Quality control:
Quality control strains were inoculated ,and cultured at 36 ± 1 for 24h ,results show as follows:
strain name strain code growth feature
Salmonella typhi CMCC (B) 50071 good turbid broth
Salmonella typhimurium CMCC (B) 50115 good turbid broth
Escherichia coli ATCC25922 prohibited --
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Specifications: 500g/bottle
*Supplement : SR0040 Novobiocin
HKM Company has been in Microbial Detection since 1993,
with manufacture base over 20,000 sq.m ,1200 sq.m of Microbiological Research Lab and GMP room and 300 staffs,HKM has a full range of Culture Media as below :
Dehydrated Culture Media
Granular Media
Chromegenic Media
Ready-to-use Culture Media
Triple wrapped contact plate & settle plate
Media Raw Materia
Other Microbial Detection Products
Package:500gr / bottle
packed in plastic bottles with plastic film wrapped, 20 bottles / carton,
Carton size :54.5*34.5*21 CM
Other packing also available according to customers demand.
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Guangdong HuanKai Microbial Sci. & Tech. Co., Ltd. is a national high-tech enterprise under the Guangdong Institute of Microbiology which is attached to Guangdong Academy of Sciences. Since established in 1993, HuanKai has adhered to the strategy of scientific and technological innovation and development, and has formed four product lines including microbiological testing reagents and consumables, digital laboratory instruments, physical and chemical rapid water detecting products, and high-efficiency environmentally friendly cleaning agents and disinfectant, both are with independent intellectual property rights. More than 2, 000 varieties of products are widely applied in mainland China, Hong Kong, Macao, Taiwan and Southeast Asia. Huankai has developed into an important R&D production base for domestic food and drug safety monitoring and control technologies and products.
Q1:Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
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