Escherichia Coli O157: H7 Rapid Test Kit Thermostatic Fluorescence for Nucleic Acid Isothermal Fluorescent Testing in Food

• Customization: Available
• Specific Usage: For Biological Purpose, For Microbiological
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents
• Model NO.: KJD09L
• Feature: Easy Operation
• Transport Package: Carton
• Specification: 24tests/box
• Trademark: BHK
• Origin: Guangdong, China
• HS Code: 3821000000

Product Description

Product Description

Introduction

This kit is based on loop-mediated isothermal amplification (LAMP) technology and is used for rapid detection of E. coli O157:H7 in food. The results can be directly interpreted with the help of a chromogenic indicator. Principle

Detection principle: Two pairs of special primers and Bst DNA polymerase with strand displacement activity are used to circulate a circular single-stranded structure at the primer junction at both ends of the template. The primers successfully combine with the template and perform strand displacement amplification reaction, achieving constant temperature Continuous and rapid amplification is carried out under certain conditions (60-65°C). When a color indicator is added to the reaction, the color difference between negative and positive results is significant, and the results can be interpreted directly through the color change.

 

Product features: 1. Quick and easy: Mix the enzyme, primer and other reaction components and then dry them into powder to increase the stability of the reagents, reduce the solution preparation steps when using, and be more convenient. The entire detection reaction can be completed within 1 hour; 2. High specificity: Design multiple primers using multiple independent regions on the target gene sequence to ensure the high specificity of the amplification reaction; 3. High sensitivity: The minimum detection limit reaches 29 CFU/Test; 4. Simple interpretation: Add a color indicator before the reaction, observe the color change of the reaction solution by naked eyes after the reaction, or track the change of the fluorescence spectrum through a fluorescence monitoring instrument to interpret the results. It is intuitive and convenient, and can effectively avoid aerosol contamination caused by opening the lid after the reaction.

 

Package contains

NO.			Product	Specification &Quantity
DRL09			Detection reagent (lyophilized)	3 rows × 8 tubes
FRL04			Reconstitution solution	650μL × 1 tube
PRL09			Positive control (lyophilized)	1 tube
SHR01			Negative control (lyophilized)	1 tube
SHR02			Lysis solution	1mL × 1 tube
SHR03			Color indicator	80μL × 1 tube
SHR04			Ultrapure water	1mL × 1 tube
			Instruction	1 pc

How to use?
1. Sample pretreatment A. Preparation of sample enrichment broth template DNA: a) When testing E. coli O157:H7 in food, aspirate 1 mL of enrichment broth into a 1.5 mL sterile centrifuge tube, centrifuge at 6000 r/min for 5 min, discard the supernatant, suspend and wash the precipitate 1-2 times with 50 μL sterile ultrapure water, centrifuge at 6000 r/min for 5 min, and completely remove the supernatant; b) Add 30 μL lysis buffer, fully suspend the bacteria, tap the tube wall to eliminate bubbles, and heat at 99 for 10 min; c) Centrifuge at 12000 r/min for 15 min. The supernatant is the crude DNA, which can be transferred to a 0.2 mL sterile centrifuge tube and can be stored for a long time at -20. B. Preparation of template DNA for suspicious colonies: Pick suspicious colonies and suspend them in a sterile centrifuge tube containing 30 μL lysis solution, and then follow the above steps b) and c). 2. Reconstitution of negative, positive controls and dry powder reagents A. Reconstitution of negative and positive controls: When used for the first time, add 80 μL of ultrapure water to the positive control and negative control freeze-dried powders, mix them after dissolving, and store them at -20 for later use. B. Reconstitution of dry powder reagents: Take out the freeze-dried powder reagents, cut n test reagent tubes as needed (n=number of samples to be tested + 1 tube of positive control + 1 tube of negative control), add 20 μL of reconstitution solution to each tube, and dissolve them completely for later use. 3. Amplification reaction A. Turn on the instrument that can provide constant temperature conditions and set it to 64 for standby; B. Add 2.5 μL of color indicator to each of the n reaction tubes that have been added with the reconstitution solution; C. Add 2.5 μL of the sample nucleic acid to be tested, the negative control, and the positive control to the n reaction tubes that have been added with the reconstitution solution, and cover the lids tightly; D. Place all the reaction tubes in the constant temperature instrument and react for 50 minutes. 4. Result interpretation: After the reaction is completed, it is recommended to place the sample on a white background under sufficient natural light to observe the results. The negative control test reagent tube is light orange, and the positive control test reagent tube is fluorescent green. The test sample reaction tube is interpreted as a control. If any result of the positive and negative control reaction tubes does not match the above situation, the test result is invalid and should be retested or contact product technical support.

Specification

Our service

1.Offering Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.

Packing & Delivery

To better ensure the safety of your goods, professional, environmentally friendly, convenient and efficient packaging services will be provided.

Company Profile

 

Guangdong HuanKai Microbial Sci. & Tech. Co.,Ltd. is a national high-tech enterprise under the Guangdong Institute of Microbiology which is attached to Guangdong Academy of Sciences. Since established in 1993, HuanKai has adhered to the strategy of scientific and technological innovation and development, and has formed four product lines including microbiological testing reagents and consumables, digital laboratory instruments, physical and chemical rapid water detecting products, and high-efficiency environmentally friendly cleaning agents and disinfectant, both are with independent intellectual property rights. More than 2, 000 varieties of products are widely applied in mainland China, Hong Kong, Macao, Taiwan and Southeast Asia. Huankai has developed into an important R&D production base for domestic food and other industry.

FAQ

1. Who are we?

HuanKai are based in Guangdong, China, start from 1993,there are total about 301-500 people in HKM. HuanKai has successfully established four major product lines: microbiological testing reagents and consumables, digital laboratory instruments, rapid water testing products for physical and chemical analysis, and eco-friendly cleaning and disinfection solutions. These lines boast over 2,000 different products, each protected by independent intellectual property rights.

2. How can we guarantee quality?
Always a pre-production sample before mass production;
Always final Inspection before shipment;

3.What can you buy from us?
Microbial Detection Product,Peptone&Tryptone,Deydrated Culture Media,Granular Media,Microbial Air Sampler and so on.

4. Why should you buy from us not from other suppliers?
1.With strong R&D capacity and good scientific research conditions,over 30 years of experience 2.One of the largest physicochemical reagent and culture media manufactuer in China follow international standard 3.Stable high quality main raw material from Europe & strictly quality control

5. What services can we provide?
Accepted Payment Currency:USD,EUR,CNY;
Accepted Payment Type: T/T,MoneyGram,PayPal,Western Union,Cash,Escrow;
Language Spoken:English,Chinese