| Customization: | Available |
|---|---|
| Certification: | Aoac |
| Color: | Yellow |
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CRM009 Vibrio Cholerae Chromogenic Medium
(Chromogenic Vibrio Cholerae Agar)
Usages:For separation and identification of Vibrio cholera in aquatic products and food poisoning sample.
Principle: Peptone and yeast extract powder to provide a nitrogen source, vitamins, amino acid and a carbon source; sucrose fermentable sugars; most non-bacteriostatic agents inhibit Vibrio cholerae bacteria, sodium chloride osmotic pressure balance can be maintained; agar as culture medium coagulant; pigment and Vibrio cholerae enzyme has specific reaction, hydrolysis of the substrate, the release of the color groups to produce yellowish green on the plate - blue-green colonies.
Formulation (per liter):
Peptone: 15g
Yeast extract powder:5g
Sucrose: 10g
Sodium chloride:5g
Bacteriostatic agent :5g
Agar :13g
Pigment:3.1g
Final pH 9.2 ± 0.1
How to use:
1, take 56.1 g of powder, dissolved in 1000ml of distilled or purified water, according to the proportion of the amplified or reduced. Heated to boiling stirring until completely dissolved, without autoclaving, cooled to about 50 , pour into sterile petri dish.
2, take 25 g (mL) of test sample with aseptical procedure, adding 225ml of alkaline peptone water , with a rotating blade homogenizer at 8 000 r / min homogenized 1 min, or slap slap type homogenizer 2 min, to prepare a uniform dilution 1:10. If no homogenizer, then samples were placed in a sterile mortar and ground, and then placed in 500 mL of sterile container, add 225 mL of alkaline peptone water and thoroughly shaken.
3, enrichment: 1:10 dilution above were cultured in 36 ± 1 6h ~ 8h, if necessary secondary enrichment.
4, the separation
take part of enrichment broth with inoculation loop ,cross and separation on Vibrio cholerae plate and incubated at 36 ± 1 18 h ~ 24 h.
5, further confirmation of Vibrio cholerae hypothetical validation test, such as oxidase, Gram staining.
Quality control:
This product appears light yellow after pouring into plate, these strains were inoculated after 36 ± 1 cultured for 18-24 hours growth in the following table:
Bacteria name inoculum (cfu / plate) growth situation feature
Vibrio cholerae non-VBO 01 30--300 good green - blue and green
Escherichia coli ATCC 25922 5000 not growth --
Vibrio vulnificus ATCC27562 5000 not growth --
Note: The additional tests for final identification need to be done.
Appearance:
Dehydrated Culture Media: light yellow powder with liquidity.
Good preparation tablet: yellow plastic.
Storage conditions and shelf life:
Dehydrated Culture Media: 10-30 , the shelf life of two years.
Prepared Medium Plate: 2-8 , save up to two weeks.
Results to determine
Color of colonies screening of microorganisms
Green - blue-green colonies Vibrio cholerae
colorless colonies other
Package:
1000ml / bottle
Other packing also available according to customers demand.
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Q1:Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
SKYPE:hkm-china
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