| CAS No.: | 075040 |
|---|---|
| Formula: | 075040 |
| EINECS: | Chemical Reagents |
| Classification: | Biochemical Reagents |
| Grade: | Br |
| Specific Usage: | For Biological Purpose, For Microbiological, Technical Grade, Pratical Use |
| Customization: |
|---|
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Usages:
For biochemical identification of microorganisms.
Instructions of biochemical identification tube
1. Opening of vial
Before opening the vials, surface disinfection with 75% alcohol cotton . Then open the plastic cover with direction of the arrow , and then open aluminum cover under sterile conditions .
2. Using of biochemical identification tube
Colonies were picked to be tested with Gram staining, fresh colony was inoculated in ordinary broth and culture at 37 for 18-24h or diluted with 0.85% sterile saline to 0.5McFa-rland (about 108cfu / mL), draw 0.05-0.08mL (about 1 to 2 drops) of broth or bacterial suspension,and added into micro-resistant bottle. Then put the rubber plug, generally using full-stopper (shown in Figure 2 bottles left), special circumstances in the following table indicate the semi-stoppered (shown in Figure 2 the right two bottles), put the vial in the Neto groove (Figure 2), or place in a suitable bottle holder and incubated at 35-37 .
The results observed in Table 1 below.
Note: For special vaccination way, incubation time or training methods, please refer to instructions included with each biochemical identification tube.
Our Services
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Q2:
After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:
Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:
During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
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