PCR Detection Kit for Escherichia Coli O157: H7 (Fluorescent Probe Assays) PCR Reagent

• Customization: Available
• CAS No.: N/a
• Formula: N/a
• Model NO.: FZ009BF2
• EINECS: N/a
• Classification: Biochemical Reagents
• Grade: Br
• Specific Usage: For Biological Purpose, For Microbiological, Technical Grade, Pratical Use
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents
• Source: Extractive
• Habit Appellation: PCR Detection Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture
• Property: Biochemical Reagent
• Transport Package: N/a
• Specification: 48tests/box
• Trademark: BHK
• Origin: China
• HS Code: 3926909090
• Production Capacity: 500 Boxes/Week

Product Description

Product Description

This kit is suitable for the detection of Escherichia coli O157:H7 in food.

Detection principle

Based on real-time PCR technology, primers, fluorescent probes and other reagents for Escherichia coli O157:H7 specific genes were added to the samples for amplification. In the process of amplification, the fluorescent probe is bound to the target gene fragment, which can be decomposed by Taq enzyme and produce a fluorescent signal. At this time, the fluorescence quantitative PCR instrument can identify the fluorescence signal, and draw the corresponding real-time amplification curve according to its strength change, so as to determine whether Escherichia coli O157:H7 is detected.

Storage

-20ºC dark storage, valid for 12 months, avoid repeated freezing and thawing.

Sensitivity:
Minimum Test limit of 100 CFU/Test


Other required materials and applicable instruments:
fluorescent quantitative PCR instrument (with fluorescent channel capable of detecting FAM label), high-speed centrifuge, pipette, pipette tip and centrifuge tube, etc
Company information
Guangdong HuanKai Microbial Sci. & Tech. Co., Ltd. is a national high-tech enterprise under the Guangdong Institute of Microbiology which is attached to Guangdong Academy of Sciences. Since established in 1993, HuanKai has adhered to the strategy of scientific and technological innovation and development, and has formed four product lines including microbiological testing reagents and consumables, digital laboratory instruments, physical and chemical rapid water detecting products, and high-efficiency environmentally friendly cleaning agents and disinfectant, both are with independent intellectual property rights. More than 2, 000 varieties of products are widely applied in mainland China, Hong Kong, Macao, Taiwan and Southeast Asia.