| CAS No.: | Hcm004 |
|---|---|
| Formula: | Bglb |
| EINECS: | 3 Years |
| Classification: | Biochemical Reagents |
| Grade: | Br |
| Specific Usage: | For Biological Purpose, For Microbiological, Technical Grade, Pratical Use |
| Customization: |
|---|
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Audited Supplier HCM004 Brilliant Green Lactose Bile Broth (BGLB)
Usages:
For determination of coliform confirmatory test for multi-tube fermentation.
Principle:
Peptone provide carbon and nitrogen sources; lactose as fermentable sugars; ox bile powder and brilliant green inhibit the non-Enterobacteriaceae.
Formulation (per liter):
Peptone: 10g
Lactose: 10g
Ox bile powder: 20g
Brilliant green: 0.0133g
Final pH7.2 ± 0.2
How to use:
1. Suspend 40g of the product, add 1 L of distilled water, stirring heated to boiling until completely dissolved, packed in a test tube with a small inverted tube, autoclave at 121 for 15min, cool to room temperature and set aside.
2.Sample handling and dilution. Slightly.
3.Transplanted LST broth tube to brilliant green lactose bile broth tube by using a inoculating loop.
4.the tube was placed in an incubator culture at 36 ± 1 for 24h.
5.Observe the results. Press production trachea number, check table report MPN MPN value per gram (mL) sample of coliform bacteria.
Quality control:
Quality control strains were inoculated and culuture at 36 ± 1 for 24h ,results are as follows:
Bacterial Name Bacterial No. Growth Status Gassing
Escherichia coli ATCC25922 good +
Salmonella typhimurium CMCC (B) 50115 good --
Staphylococcus aureus ATCC6538 inhibited --
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Specifications: 500g
HKM Company has been in Microbial Detection since 1993,
with manufacture base over 20,000 sq.m ,1200 sq.m of Microbiological Research Lab and GMP room and 300 staffs,HKM has a full range of Culture Media as below :
Dehydrated Culture Media
Granular Media
Chromegenic Media
Ready-to-use Culture Media
Triple wrapped contact plate & settle plate
Media Raw Materia
Other Microbial Detection Products
Package:
500gr / bottle :Packed in plastic bottles with plastic film wrapped, 20 bottles / carton,
Carton size :54.5*34.5*21 CM
Other packing also available according to customers demand.
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Q1:
Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:
After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:
Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:
During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
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