Mannito Salt Agar Chapman Medium for Isolation of Presumptive Pathogenic Staphylococci

• Appearance: Powder
• Material: Powder
• Shelf Life: 3 Years
• Feature: Light Proof Packaging
• Customized Support: OEM
• Colour: Light Yellow
• Model NO.: HCM045
• Transport Package: Carton
• Specification: 500g/bottle
• Trademark: HKM
• Origin: Guangdong, China
• HS Code: 3821000000

Product Description

Product Description

HKM Company has been in Microbial Detection since 1993, with manufacture base over 20,000 sq.m ,1200 sq.m of Microbiological Research Lab and GMP room and 300 staffs,HKM has a full range of Culture Media as below :

Dehydrated Culture Media

Granular Media

Chromegenic Media

Ready-to-use Culture Media

Triple wrapped contact plate & settle plate

Media Raw Materia

Other Microbial Detection Products

Principle

Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ;phenolsulfonphthalein
as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.

Formulation(per liter)
Pancreatic digest of casein 5.0g
Pancreatic digest of animal tissue 5.0g
Beef Extract 1.0g
Sodium Chloride 75.0g
Mannitol 10.0g
Phenol Red 0.025g
Agar 15.0g
Final PH 7.4±0.2


How to use
1.Suspend 111g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.

 

Specification

Item	Value
Form	Powder
Packaging	Bottle, Drum
Place of Origin	China, Guangdong
Brand Name	HKM CHINA
Model Number	HCM045
Application	For selective isolation and culture of Staphylococcus aureus
Reagent grade	Biochemical Reagent
Physical behavior	Powder
Appearance	light yellow powder

Quality control

Packing & Delivery

Package:500g / bottle :Packed in plastic bottles with plastic film wrapped, 20 bottles / carton.
Carton size :55*35*21 cm
Other packing also available according to customers demand.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

To better ensure the safety of your goods, professional, environmentally friendly, convenient and efficient packaging services will be provided.

Our service

1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.

Company Profile

Guangdong HuanKai Microbial Sci. & Tech. Co.,Ltd. is a national high-tech enterprise under the Guangdong Institute of Microbiology which is attached to Guangdong Academy of Sciences. Since established in 1993, HuanKai has adhered to the strategy of scientific and technological innovation and development, and has formed four product lines including microbiological testing reagents and consumables, digital laboratory instruments, physical and chemical rapid water detecting products, and high-efficiency environmentally friendly cleaning agents and disinfectant, both are with independent intellectual property rights. More than 2, 000 varieties of products are widely applied in mainland China, Hong Kong, Macao, Taiwan and Southeast Asia. Huankai has developed into an important R&D production base for domestic food and other industry.

FAQ

Q1:Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.

Q2: After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
The reason may be: The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after
high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.

Q3: Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β-glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other
methods.

Q4:During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.

 

5. Who are we?
We are based in Guangdong, China, start from 1993,sell to Domestic Market(80.00%),Southeast Asia(5.00%),Mid East(5.00%),Africa(3.00%),South America(2.00%),North America(1.00%),Eastern Europe(1.00%),South Asia(1.00%),Central America(1.00%),Western Europe(1.00%). There are total about 301-500 people in our office.

6. How can we guarantee quality?
Always a pre-production sample before mass production;
Always final Inspection before shipment;

7.What can you buy from us?
Microbial Detection Product,Peptone&Tryptone,Deydrated Culture Media,Granular Media,Microbial Air Sampler and so on.

8. Why should you buy from us not from other suppliers?
1.With strong R&D capacity and good scientific research conditions,over 30 years of experience 2.Largest culture media manufactuer in China follow international standard 3.Stable high quality main raw material from Europe & strictly quality control

9. What services can we provide?
Accepted Payment Currency:USD,EUR,CNY;
Accepted Payment Type: T/T,MoneyGram,PayPal,Western Union,Cash,Escrow;
Language Spoken:English,Chinese