| Customization: | Available |
|---|---|
| Classification: | Biochemical Reagents |
| Grade: | Br |
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Product Description
MRS Broth
Usages:
For cultivating of Lactobacillus species.
Principle:
Enzymatic digest of casein, beef extract powder, yeast extract powder to provide a nitrogen source, vitamins, growth factors; ammonium citrate, magnesium sulfate, manganese sulfate, Tween-80 and sodium acetate to provide the training of various lactic acid bacteria growth factor.
Formulation (per liter):
Enzymatic digest of casein 10g
Meat extract powder 10g
Yeast extract powder 4g
Triammonium citrate 2g
Sodium acetate 5g
Magnesium sulfate (MgSO4.7H2O) 0.2g
Manganese sulfate (MnSO4.4H2O) 0.05g
Dipotassium hydrogen phosphate 2g
Glucose 20g
Tween-80 1.08g
Final pH 5.7 ± 0.2
How to use:
1. Suspend 54g in 1 L of distilled or deionized water ,heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 for 15min.
2.Dilluted and treated samples.
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Specifications: 250g/bottle
Packing material:Packed in plastic bottles with plastic film wrapped
Capacity:250gr /botte ,20 bottle/ carton .
10KG / 25Kg is also available
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Q1:
Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:
After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:
Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:
During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
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