| Classification: | Biochemical Reagents |
|---|---|
| Grade: | Br |
| Specific Usage: | For Biological Purpose, For Microbiological |
| Content: | Standard |
| Usage: | Laboratory Reagents, Analytical Reagents, Teaching Reagents |
| Source: | Dry Powder |
| Customization: |
|---|
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PRODUCT: HCM089 R2A Agar
Usages: For enumeration of heterotrophic bacteria in water.
Principle
Yeast extract, peptone, provide nitrogen acid protein hydrolysates, vitamins, growth factors; glucose, to fermentable sugars; soluble starch can absorb the harmful substances produced by bacteria in the recovery process; sodium pyruvate may enhance recovery of bacteria. PH buffer is potassium dihydrogen phosphate; provided over anhydrous magnesium divalent cations; agar as coagulant.
Formulation(per liter)
Yeast extract powder :0.5g
Peptone: 0.5g
Casein hydrolyzate: 0.5g
Glucose: 0.5g
Soluble starch :0.5g
Potassium dihydrogen phosphate :0.3g
Anhydrous magnesium sulfate :0.024g
Sodium pyruvate :0.3g
Agar :15.0g
Final pH : 7.2 ± 0.2
How to use
1.Suspend 18.1g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Quality control
|
The name and number of strain |
Indicator |
Culture Time & Temperature |
Colony |
|
Bacillus subtilis CMCC(B)63501 |
Promote growth ability |
24h,30-35 Good |
PR≥0.7,white colony
|
|
Pseudomonas aeruginosa CMCC(B)10104 |
Package: Packing material:Packed in plastic bottles with plastic film wrapped
Capacity: 20 bottle/ carton Carton size :54.5*34.5*21CM
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Guangdong HuanKai Microbial Sci. & Tech. Co., Ltd. is a national high-tech enterprise under the Guangdong Institute of Microbiology which is attached to Guangdong Academy of Sciences. Since established in 1993, HuanKai has adhered to the strategy of scientific and technological innovation and development, and has formed four product lines including microbiological testing reagents and consumables, digital laboratory instruments, physical and chemical rapid water detecting products, and high-efficiency environmentally friendly cleaning agents and disinfectant, both are with independent intellectual property rights. More than 2, 000 varieties of products are widely applied in mainland China, Hong Kong, Macao, Taiwan and Southeast Asia. Huankai has developed into an important R&D production base for domestic food and drug.
Q1:Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be: The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3: Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
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