| Customization: | Available |
|---|---|
| CAS No.: | 2 Years |
| Formula: | Salmonella Test Kit |
Suppliers with verified business licenses
Audited Supplier Audited by an independent third-party inspection agency
Biochemical Identification Kit of Bacteria Instructions
--071 740 biochemical identification of Salmonella box (10 kinds × 10 times)
I) package of reagents
ten kinds of biochemical reaction reagents (Table 1), 10 bottles of 0.85% sterile saline, 1 bottle of 0.5 McFarland turbidity tube, 10 Pcs of disposable pipette.
II) using method of ampoule
Before opening of ampoule, disinfectanting the surface with 75% alcohol cotton, then open the aluminum cover under sterile conditions according to the direction of the arrow .aluminum cover ripped open the vial stopper (Figure 1). All the ampoule can be threw away after use and being autoclaving discarded.
Note: when using potassium cyanide biochemical tube, the rubber stopper should be set tight to avoid false positives caused by potassium cyanide volatilization! (If you find the color of potassium cyanide biochemical identification tube turns from pale yellow sepia
Or black then mean it is bad and can not continue to use)
III) the using method of Salmonella biochemical identification box
Salmonella is aerobic gram-negative,non-bacillus , with flagella the optimum temperature is 37 , the optimum pH is 7.2 to 7.6.
On solid medium culture at 37 for 24h colonies showed a medium size, smooth, round, moist, uplift, uniformly turbid growth in liquid medium. Picked suspicious bacteria directly and cultivate on triple sugar iron agar and nutrient agar, then place suspected colonies into a sterile saline ,compare with the 0.5 McFarland turbidity tube ,prepared the 0.5McFarland (about 108cfu / mL), Pipette 2 drops (about 0.06mL) bacterial suspension and add to each micro biochemical tube. Put the rubber plug on the ampoule after inoculation, generally it is all-stopper (shown in Figure 2 bottles left), and semi-stoppered (Figure 2 bottles shown right)is necessary for some special situation, standing in the Neto groove (Figure 2), or placed in a suitable bottle rack, and cultured at 35-37 in an incubator, then observe the results show in Table 1.
Note: If there are special way of inoculation, incubation time or training, please see the instructions that came with each biochemical identification tube.
Table 1 Biochemical Identification of Salmonella
|
Product Name |
results to determine |
incubation time(hours) |
Instruction |
|
|
Positive |
Negative |
|||
|
Triple sugar iron agar |
Produce acid and turn yellow Produce hydrogen sulfide and turn black |
discoloration |
24 |
Pick the colony with inoculation loop,after the completion of the puncture on a slant zoned "" zigzag inoculation, semi-stoppered ampoule (bottles as shown right side in Figure 2) erection of cultivation.
|
|
Peptone water |
red |
discoloration |
24 |
add 2-3 drops of indole reagent after cultultivation ,semi-stoppered ampoule (bottles as shown right side in Figure 2) and immediately observe the results.
|
|
urea |
pink pale |
orange-red |
12-24 |
|
|
Potassium cyanide |
growth |
not growth |
24-48 |
test tubes and control tubes were positive for growth; test tube does not grow, control tube growth was negative.
|
|
Lysine decarboxylase |
Test tube become blue-green, Control tube become yellow
|
Test tube and control tube become yellow
|
18-24 |
per plant was seized amino acid bacteria should also be vaccinated a control tube and add sterile liquid paraffin covering the surface of the medium 3-4 drops. Cultured for 18-24 hours, no positive result, continue to train 4 days, then do a final determination through result.
|
|
Mannitol, sorbitol |
yellow |
purple |
8-24 |
|
|
β- galactosidase (ONPG)) |
yellow |
light yellow or colorless |
12-24 |
|
Note: The reagent is placed in the order of HKM catalog from left to right .
Packing material: 10types for 10 tests
Details:
1.075750 triple sugar iron agar
2.075240 peptone water
3.075170 urea
4.07330 potassium cyanide(KCN) growth test tube
5.075340 potassium cyanide(KCN) growth control tube
6. 075280 lysine decarboxylase
7. 075290 amino acid decarboxylase
8. 075040 mannitol
9. 075090 sorbitol
10. 075180 ONPG B- galactosidase (ONPG)
Additives Reagent
029030 kovacs indole reagent
029110 Liquid paraffin
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Q1:
Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:
After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:
Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:
During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
){kind=link}