| Customization: | Available |
|---|---|
| Specific Usage: | For Biological Purpose, For Microbiological |
| Content: | Standard |
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HCM032 Kligler Iron Agar
Usages: For differentiation of enterobacteriaceae.
HKM Company has been in Microbial Detection since 1993, with manufacture base over 20,000 sq.m ,1200 sq.m of Microbiological Research Lab and GMP room and 300 staffs,HKM has a full range of Culture Media as below :
1.Dehydrated Culture Media
2.Granular Media
3.Chromegenic Media
4.Ready-to-use Culture Media
5.Triple wrapped contact plate & settle plate
6.Media Raw Materia
7.Other Microbial Detection Products
Principle:Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.
Formulation(per liter)
Peptone 20g
Beef extract powder 3g
Yeast extract powder 3g
Lactose 10g
Dextrose 1g
Sodium chloride 5g
Ferric ammonium citrate 0.5g
Sodium thiosulfate 0.5g
Agar 12g
Phenol red 0.05g
Final pH 7.4 ± 0.2
How to use?
1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Quality control
Item The name and number of strain Growth Colony Color
1 Escherichia coli ATCC25922 Good A/A
2 Proteus CMCC (B) 49027 Good K/A
3 Salmonella typhimurium CMCC (B) 50115 Good K/A
"A"Acid , "K" Alkality
Package:500g / bottle
Packed in plastic bottles with plastic film wrapped, 20 bottles / carton
Carton size :54.5*34.5*21 CM
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other packing also available according to customers demand.
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Guangdong HuanKai Microbial Sci. & Tech. Co., Ltd. is a national high-tech enterprise under the Guangdong Institute of Microbiology which is attached to Guangdong Academy of Sciences. Since established in 1993, HuanKai has adhered to the strategy of scientific and technological innovation and development, and has formed four product lines including microbiological testing reagents and consumables, digital laboratory instruments, physical and chemical rapid water detecting products, and high-efficiency environmentally friendly cleaning agents and disinfectant, both are with independent intellectual property rights. More than 2, 000 varieties of products are widely applied in mainland China, Hong Kong, Macao, Taiwan and Southeast Asia. Huankai has developed into an important R&D production base for domestic food and drug.
Q1:Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
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