Modified Lauryl Sulfate Tryptose Broth (MLST) for Enriching Enterobacter Sakazakii Molecular Detection

Product Description

Product Description

HCM037 Modified Lauryl Sulfate Tryptose Broth Base (MLST)

Usages:
For enrichment and molecular biological test methods of Enterobacter sakazakii in milk powder.

Principle:
Tryptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride maintains osmotic equilibrium; lactose is coliform fermentable sugars; potassium dihydrogen phosphate and dipotassium hydrogen phosphate is a buffer; lauryl sodium and van-comycin inhibit the growth of Gram-positive bacteria.

Formulation(per liter):
Trypticase 20.0g
Sodium chloride 34.22g
Lactose 5.0g
Potassium dihydrogen phosphate 2.75g
Dipotassium hydrogen phosphate 2.75g
Sodium lauryl sulfate 0.1g
Final pH6.8 ± 0.2

How to use:
1.Suspend 64.8g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 500g/bottle
*Supplement: SR0180 van-comycin

 

HKM Company has been in Microbial Detection since 1993,

with manufacture base over 20,000 sq.m ,1200 sq.m of Microbiological Research Lab and GMP room and 300 staffs,HKM has a full range of Culture Media as below :

 

Dehydrated Culture Media 

 

Granular Media 

 

Chromegenic Media 

 

Ready-to-use Culture Media 

 

Triple wrapped contact plate & settle plate

 

Media Raw Materia 

 

Other Microbial Detection Products

 

Packaging & Shipping

 Package:

500gr / bottle :Packed in plastic bottles with plastic film wrapped, 20 bottles / carton,

 

Carton size :54.5*34.5*21 CM

 

Other packing also available according to customers demand.

Our Services

 

1.Offering Free Sample for Testing .

2.R & D team to provide technical support.

3.Provide packaging, labeling OEM customization. 

 

Company Information

 

FAQ

 

Q1:

Sometimes color of dehydrated culture media  between batches have subtle differences,would this affect test results?

A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.

 

Q2:

After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?

A2: The reason may be:

The required hydration in the preparation process have not been done completely .That is to say   distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.

 

Q3:

Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?

A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.

 

Q4:

During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?

A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.