| Customization: | Available |
|---|---|
| Classification: | Biochemical Reagents |
| Grade: | Br |
Suppliers with verified business licenses
Audited Supplier Audited by an independent third-party inspection agency
Linden Grain Medium
Usages:
For detection of molds, yeasts and total bacteria , commonly used in aseptic filling empty PET bottle production line of sterility testing.
Principle: peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; glucose carbon source; potassium dihydrogen phosphate as a buffer; provide essential trace elements of magnesium sulfate; ammonium sulfate osmotic equilibrium can be maintained and conducive to the growth target bacteria
Formulation (per liter):
Glucose :20.0g
Ammonium sulfate:2.0g
Magnesium sulfate:1.0g
Peptone:2.0g
Yeast extract powder: 3.5g
Sodium dihydrogen phosphate: 1.0g
Final pH 6.5 ± 0.2
How to use:
1. Weight 29.5g of product, adding 1L of distilled water or deionized water,stirring heated to boiling until complete dissolution, dispensing tube or flask, 121 sterilization for 15minutes.
2. Inoculated the treated sample to LG medium, according to the specific requirements of sterility testing, vaccination appropriate sample size, the number of pieces of medium and positive control, the medium was placed in 28 ~ 32 prescribed time training 13 ~ 15d.
3. Observe the results.
Quality Control:
Appearance:
Powder: light yellow powder
Prepared broth: yellow, clear and transparent without precipitation.
Biology testing: The following strains were inoculated after 28 ~ 32 cultured 13 ~ 15d growth in the following table:
Quality control
Bacteria Name Bacteria No. growth characteristics
Pseudomonas aeruginosa ATCC27853 turbid broth
Staphylococcus aureus ATCC6538 turbid broth
Bacillus cereus ATCC63303 turbid broth
Klebsiella pneumonia ATCC46117 turbid broth
Hyphae of Aspergillus niger ATCC16404 flocculent, liquid black spores can
Candida albicans ATCC10231 turbid broth
Specifications: 10kg / bag,20kg / bag
Storage: Store in a dark, cool and dry place,sealed the product immediately after use.
Storage period of three years
Specifications: 10kg / bag,20kg / bag
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Q1:
Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:
After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:
Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:
During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
){kind=link}