| Classification: | Biochemical Reagents |
|---|---|
| Grade: | Br |
| Specific Usage: | For Biological Purpose, For Microbiological, Technical Grade, Pro Analysis |
| Content: | Standard |
| Usage: | Laboratory Reagents, Analytical Reagents, Teaching Reagents |
| Source: | Dry Powder |
| Customization: |
|---|
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PRODUCT: HCM046 Vogel Johnson Agar
Usages:
For selective isolation of staphylococcus aureus.
Principle:
Peptone and beef extract powder provides carbon, nitrogen, vitamins and minerals; D- mannitol to fermentable sugars; higher levels of sodium chloride to provide a higher osmotic pressure, suppress most non-staphylococcal microorganisms ; phenolsulfonphthalein as pH indicator; agar is medium coagulant. Typical pathogenic staphylococci (coagulase positive) D- mannitol produce acid fermentation and produce yellow colonies with a yellow halo, typically non-pathogenic Staphylococcus unfermented D- mannitol to form red colonies.
Formulation(per liter):
|
Pancreatic Digest of Casein |
10g |
|
Yeast Extract |
5 g |
|
Mannitol |
10 g |
|
Dibasic Potassium Phosphate |
5 g |
|
Lithium chloride |
5 g |
|
Glycine |
10 g |
|
Agar |
16 g |
|
Phenol Red |
25mg |
|
Final pH |
7.2±0.2 |
How to use:
1.Suspend 61g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Quality control:
|
Item |
The name and number of strain |
Growth |
Colony Color |
|
1 |
Staphylococcus aureus CMCC (B) 26003 |
Good |
Golden yellow |
|
2 |
Staphylococcus epidermidis CMCC (B) 26069 |
Good |
Red |
|
3 |
Escherichia coli CMCC (B) 44102 |
Inhibition |
-- |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle
Package:Packing material:Packed in plastic bottles with plastic film wrapped
Capacity: 20 bottle/ carton Carton size :54.5*34.5*21CM
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Q1:
Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:
After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:
Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
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