Enterobacter Cloacae Isolation Agar Sewer Enterobacter separation Medium for Selective Isolation and Culture

• Color: Yellow
• Function: Microbial Testing
• Appearance: Powder
• Material: Powder
• Feature: Light Proof Packaging
• Customized Support: OEM
• Model NO.: 028700
• Colour: Light Yellow
• Transport Package: Carton
• Specification: 250g/bottle
• Trademark: HKM
• Origin: Guangdong, China
• HS Code: 3821000000

Product Description

Product Description

HKM Company has been in Microbial Detection since 1993, with manufacture base over 20,000 sq.m ,1200 sq.m of Microbiological Research Lab and GMP room and 300 staffs,HKM has a full range of Culture Media as below :

Dehydrated Culture Media

Granular Media

Chromegenic Media

Ready-to-use Culture Media

Triple wrapped contact plate & settle plate

Media Raw Materia

Other Microbial Detection Products

Principle:
Peptone, beef extract powder provides carbon, nitrogen, vitamins and minerals; lactose, sucrose and salicin to fermentable sugars; bile salts, sodium deoxycholate, bromothymol blue and acidic fuchsin suppression gram-positive bacteria; sodium chloride to maintain osmotic equilibrium; sodium thiosulfate and ferric ammonium citrate for the detection of hydrogen sulfide, so that the center of black colonies; agar is medium coagulant; bromothymol blue and acid fuchsin as pH indicator.

Formulation(per liter)
Peptone 12g
Beef extract powder 3g
Lactose 12g
Sugar 2g
Salicin 9g
Bile salt 9g
Sodium chloride 5g
Agar 14g
Bromothymol blue 0.065g
Acid fuchsin 0. 1g
Sodium thiosulfate 5g
Ferric ammonium citrate 1.5g
Final pH7.5 ± 0.2

How to use
1.Suspend 72.7g in 1 L of distilled water , stirring heated to boiling and pour into sterile plates. Do not autoclave this medium.
2.Diluted and treated samples.

Quality control
Item The name and number of strain Growth Colony Color
1 Salmonella typhimurium CMCC (B) 50115 Good Light green-blue
2 Sonnei Shigella bacteria CMCC (B) 51592 Good Green-blue
3 Escherichia coli ATCC25922 Partial Inhibition --

 

Specification

Item	Value
Form	Powder
Packaging	Bottle, Drum
Place of Origin	China, Guangdong
Brand Name	HKM CHINA
Model Number	028700
Application	Others
Reagent grade	Biochemical Reagent
Physical behavior	Powder
Appearance	light yellow powder

Packing & Delivery

Package:250g / bottle :Packed in plastic bottles with plastic film wrapped, 20 bottles / carton.
Carton size : 57*33*17cm
Other packing also available according to customers demand.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

To better ensure the safety of your goods, professional, environmentally friendly, convenient and efficient packaging services will be provided.

Our service

1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.

Company Profile

Founded in 1993, Guangdong Huankai Microbial Sci. & Tech. Co.,Ltd. is the most important manufacturing and R&D base in the field of detection and control of food safety in China. It is an high-tech corporation belonging to The Guangdong Institute of Microbiology. Through undertaking National and Provincial Level Food safety projects it has won 12 National and Guangdong Provincial level Awards, and has been awarded 33 National patents. In recent years it has been actively participating in the formulation of National and Trading microbial hygiene standards for food and mineral water.

Huankai has always been devoted to monitoring biological safety and controlling the development, production and management of products. At the present it produces microbial monitoring products on a large scale, consisting of 3 large ranges, which amount to

nearly 600 varieties of product, including: a series of rapid water quality detection products, highly efficient and environmentally friendly disinfectants as well as biologocial safety laboratory equipment. All of which are largely used in provinces and cities around China, Hong Kong, Macao, Taiwan, as well as other East Asian Countries. After 10 years of development, Huankai has already become the largest manufacturer of microbial monitoring products in China, and the largest manufacturer of industrial cleaning detergent in South China.With its R&D production bases located in Guangzhou Science City Guangdong Advanced Bio-Tech Demonstration Zone, Huankai now has industry leading GMP workshops producing microbial detection products as well as the largest production line in South China for highly efficient environmentally friendly industrial cleaning detergents used in the food and medical sector. These advanced conditions are helping Huankais speedy development.

FAQ

Q1:Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.

Q2: After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
The reason may be: The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after
high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.

Q3: Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β-glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other
methods.

Q4:During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.

 

5. Who are we?
We are based in Guangdong, China, start from 1993,sell to Domestic Market(80.00%),Southeast Asia(5.00%),Mid East(5.00%),Africa(3.00%),South America(2.00%),North America(1.00%),Eastern Europe(1.00%),South Asia(1.00%),Central America(1.00%),Western Europe(1.00%). There are total about 301-500 people in our office.

6. How can we guarantee quality?
Always a pre-production sample before mass production;
Always final Inspection before shipment;

7.What can you buy from us?
Microbial Detection Product,Peptone&Tryptone,Deydrated Culture Media,Granular Media,Microbial Air Sampler and so on.

8. Why should you buy from us not from other suppliers?
1.With strong R&D capacity and good scientific research conditions,over 30 years of experience 2.Largest culture media manufactuer in China follow international standard 3.Stable high quality main raw material from Europe & strictly quality control

9. What services can we provide?
Accepted Payment Currency:USD,EUR,CNY;
Accepted Payment Type: T/T,MoneyGram,PayPal,Western Union,Cash,Escrow;
Language Spoken:English,Chinese