Rapid Nucleic Acid Detection Kit for Burkholderia Gladioli in Food

• Customization: Available
• Specific Usage: For Biological Purpose, For Microbiological
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents
• Model NO.: KJD16L
• Material: Liquid
• Feature: Easy Operation
• Customized Support: OEM
• Transport Package: Carton
• Specification: 24tests/box
• Trademark: BHK
• Origin: Guangdong, China

Product Description

Product Description

Introduction

This kit is only suitable for qualitative detection of fecal streptococci in water bodies. Other tests must be carried out in accordance with the national standard "GB8538-2016 National Food Safety Standard Drinking Natural Water Detection Method". Principle

This kit is based on loop-mediated isothermal amplification (LAMP) technology and is used for the rapid detection of Burkholderia gladioli in food. The results can be directly interpreted with the help of the color change of the chromogenic indicator. . Two pairs of special primers and Bst DNA polymerase with strand displacement activity are used to circulate a circular single-stranded structure at the primer junction at both ends of the template. The primers successfully combine with the template and perform strand displacement amplification reaction, achieving constant temperature Continuous and rapid amplification is carried out under certain conditions (60~65). When a color indicator is added to the reaction, the color difference between negative and positive results is significant, and the results can be interpreted directly through the color change.

 

Product features: 1. Quick and easy: Mix the enzyme, primer and other reaction components and then dry them to increase the stability of the reagents. This reduces the steps of solution preparation during use and is more convenient. The entire detection reaction can be completed within 1 hour; 2. High specificity: Multiple primers are designed using multiple independent regions on the target gene sequence to ensure the high specificity of the amplification reaction; 3. High sensitivity: The minimum detection limit reaches 8 CFU/Test; 4. Simple interpretation: Add a color indicator before the reaction, and after the reaction, observe the color change of the reaction solution with the naked eye or track the changes in the fluorescence spectrum through a fluorescence monitoring instrument to interpret the results. This is intuitive and convenient, and can effectively avoid aerosol contamination caused by opening the lid after the reaction

 

Package contains

NO.			Product	Specification &Quantity
			Detection reagent (lyophilized)	3 rows × 8 tubes
			Reconstitution solution	650μL × 1 tube
KJD16L+			Positive control (lyophilized)	1 tube
			Negative control (lyophilized)	1 tube
			Lysis solution	1mL × 1 tube
			Color indicator	80μL × 1 tube
			Ultrapure water	1mL × 1 tube
			Instruction	1 pc

How to use?
1. Sample pretreatment A. Preparation of sample enrichment broth template DNA: a) Take 25 g of the sample to be tested and place it in 225 mL PDB enrichment broth, and enrich the bacteria at 36 ±1 for 8~12 h. This step can also be performed according to the method established by the laboratory; b) Take 1 mL of the above enrichment broth to a 1.5 mL sterile centrifuge tube, centrifuge at 6000 r/min for 5 min, and completely remove the supernatant; c) Add 30 μL lysis buffer to fully suspend the sediment at the bottom of the tube, tap the tube wall to eliminate bubbles, and heat at 99 for 10 min. d) Centrifuge at 12000 r/min for 15 min. The supernatant is the sample DNA to be tested. Leave it on ice and centrifuge it again if it is not used for a long time. B. Preparation of template DNA of suspicious colonies: Pick up a half circle of suspicious colonies and put it into a sterile centrifuge tube containing 30 μL lysis solution. After fully suspending, follow the above steps c) and d). 2. Mix reagents and add samples A. Reconstitution of negative and positive controls: When used for the first time, add 80 μL of ultrapure water to the positive control and negative control freeze-dried powder, mix after dissolving, and store at -20 for later use. B. Reconstitution and sample addition of dry powder reagent: 1) Take out the freeze-dried powder reagent, cut n test reagent tubes as needed (n = number of samples to be tested + 1 tube of positive control + 1 tube of negative control), and add 20 μL of reconstitution solution to each tube; 2) Add 2.5 μL of color indicator to each of the n reaction tubes to which the reconstitution solution has been added; 3) Add 2.5 μL of negative control, sample DNA to be tested, and positive control to the n reaction tubes in turn, and cover the lid immediately after each addition. 3. Amplification reaction: Use an instrument that can provide constant temperature conditions (preferably with a hot cover function), set the temperature to 64°C, and place the test tube in a constant temperature reaction for 50 min. 4. Result interpretation: After the reaction is completed, place the sample on a white background for observation in an environment with sufficient natural light. The negative control test tube will be light orange, and the positive control test tube will be fluorescent green. The test sample reaction tube will be interpreted based on this. If any result of the positive or negative control reaction tube does not match the above situation, the test result is invalid and should be retested or contact product technical support.

Specification

Our service

1.Offering Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.

Packing & Delivery

 

To better ensure the safety of your goods, professional, environmentally friendly, convenient and efficient packaging services will be provided.

Company Profile

 

Guangdong HuanKai Microbial Sci. & Tech. Co.,Ltd. is a national high-tech enterprise under the Guangdong Institute of Microbiology which is attached to Guangdong Academy of Sciences. Since established in 1993, HuanKai has adhered to the strategy of scientific and technological innovation and development, and has formed four product lines including microbiological testing reagents and consumables, digital laboratory instruments, physical and chemical rapid water detecting products, and high-efficiency environmentally friendly cleaning agents and disinfectant, both are with independent intellectual property rights. More than 2, 000 varieties of products are widely applied in mainland China, Hong Kong, Macao, Taiwan and Southeast Asia. Huankai has developed into an important R&D production base for domestic food and other industry.

FAQ

1. Who are we?

HuanKai are based in Guangdong, China, start from 1993,there are total about 301-500 people in HKM. HuanKai has successfully established four major product lines: microbiological testing reagents and consumables, digital laboratory instruments, rapid water testing products for physical and chemical analysis, and eco-friendly cleaning and disinfection solutions. These lines boast over 2,000 different products, each protected by independent intellectual property rights.

2. How can we guarantee quality?
Always a pre-production sample before mass production;
Always final Inspection before shipment;

3.What can you buy from us?
Microbial Detection Product,Peptone&Tryptone,Deydrated Culture Media,Granular Media,Microbial Air Sampler and so on.

4. Why should you buy from us not from other suppliers?
1.With strong R&D capacity and good scientific research conditions,over 30 years of experience 2.One of the largest physicochemical reagent and culture media manufactuer in China follow international standard 3.Stable high quality main raw material from Europe & strictly quality control

5. What services can we provide?
Accepted Payment Currency:USD,EUR,CNY;
Accepted Payment Type: T/T,MoneyGram,PayPal,Western Union,Cash,Escrow;
Language Spoken:English,Chinese