| Customization: | Available |
|---|---|
| CAS No.: | Fraser Broth Base |
| Formula: | Hcm023 |
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HCM023 Fraser Broth Base
HKM Company has been in Microbial Detection since 1993,
with manufacture base over 20,000 sq.m ,1200 sq.m of Microbiological Research Lab and GMP room and 300 staffs,HKM has a full range of Culture Media as below :
Dehydrated Culture Media
Granular Media
Chromegenic Media
Ready-to-use Culture Media
Triple wrapped contact plate & settle plate
Media Raw Materia
Other Microbial Detection Products
Usages:
For selective enrichment of Listeria spp.
Principle:
Casein digest, animal tissue enzyme digests, beef extract powder, yeast extract powder provide nitrogen, vitamins, amino acids and growth factors; sodium chloride maintains osmotic equilibrium; disodium hydrogen phosphate and potassium dihydrogen phosphate buffer agent; Esculin is fermentable sugars; lithium chloride and other antibiotics can inhibit the Gram-negative bacteria and most gram-positive bacteria.
Formulation(per liter):
Casein digest 5.0g
Animal tissue enzyme digests 5.0g
Beef extract powder 5.0g
Yeast extract powder 5.0g
NaCl 20.0g
Disodium hydrogen phosphate 12.0g
Potassium dihydrogen phosphate 1.35g
Esculin 1.0 g
Lithium chloride 3.0g
Final pH 7.2 ± 0.2
How to use:
1.Suspend 28.7g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Quality control:
Item The name and number of strain Growth Colony Color
1 Listeria monocytogenes bacteria CMCC (B) 54002 Good Black and cloudy
2 Escherichia coli ATCC25922 Inhibited --
3 Streptococcus faecalis CMCC (B) 32223 Inhibited --
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 500g/bottle
*Supplement: SR0120( acriflavine hydrochloride, nalidixic acid, ferric ammonium citrate)
SR0130 (criflavine hydrochloride, nalidixic acid)
Package:
500gr / bottle :Packed in plastic bottles with plastic film wrapped, 20 bottles / carton,
Carton size :54.5*34.5*21 CM
Other packing also available according to customers demand.
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Founded in 1993, Guangdong Huankai Microbial Sci. & Tech. Co.,Ltd. is the most important manufacturing and R&D base in the field of detection and control of food safety in China. It is an high-tech corporation belonging to The Guangdong Institute of Microbiology. Through undertaking National and Provincial Level Food safety projects it has won 12 National and Guangdong Provincial level Awards, and has been awarded 33 National patents. In recent years it has been actively participating in the formulation of National and Trading microbial hygiene standards for food and mineral water.
Huankai has always been devoted to monitoring biological safety and controlling the development, production and management of products. At the present it produces microbial monitoring products on a large scale, consisting of 3 large ranges, which amount to nearly 600 varieties of product, including: a series of rapid water quality detection products, highly efficient and environmentally friendly disinfectants as well as biologocial safety laboratory equipment. All of which are largely used in provinces and cities around China, Hong Kong, Macao, Taiwan, as well as other East Asian Countries. After 10 years of development, Huankai has already become the largest manufacturer of microbial monitoring products in China, and the largest manufacturer of industrial cleaning detergent in South China.
With its R&D production bases located in Guangzhou Science City Guangdong Advanced Bio-Tech Demonstration Zone, Huankai now has industry leading GMP workshops producing microbial detection products as well as the largest production line in South China for highly efficient environmentally friendly industrial cleaning detergents used in the food and medical sector. These advanced conditions are helping Huankais speedy development.
Q1:Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
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