Simon Citrate Identification Tube for Diagnostic of Microorganisms

• CAS No.: Simon Citrate
• Formula: Chemical Reagents
• EINECS: Biochemical
• Classification: Physicochemical Reagent
• Specific Usage: Technical Grade, Physicochemical Analysis
• Content: Standard
• Model NO.: 075200
• Usage: Laboratory Reagents, Analytical Reagents, Teaching Reagents
• Source: Physicochemical Reagent
• Habit Appellation: Physicochemical Reagent
• Application: Industry, Scientific Research
• Property: Physicochemical Reagent
• Transport Package: N/a
• Specification: 20 vials/box
• Trademark: HKM
• Origin: China
• Production Capacity: 5000 Boxes/Week

Product Description

 

 

 

Product Description

 

Usages:

 

For biochemical identification of microorganisms.

 

Instructions of biochemical identification tube

1. Opening of vial

Before opening the vials, surface disinfection with 75% alcohol cotton . Then open the plastic cover with direction of the arrow , and then open aluminum cover under sterile conditions .

2. Using of biochemical identification tube

Colonies were picked to be tested with Gram staining, fresh colony was inoculated  in ordinary broth and culture at 37 for 18-24h or diluted with 0.85% sterile saline to 0.5McFa-rland (about 108cfu / mL), draw 0.05-0.08mL (about 1 to 2 drops) of broth or bacterial suspension,and added into micro-resistant bottle. Then put the rubber plug, generally using full-stopper (shown in Figure 2 bottles left), special circumstances in the following table indicate the semi-stoppered (shown in Figure 2 the right two bottles), put the vial in the Neto groove (Figure 2), or place in a suitable bottle holder and incubated at 35-37 .

The results observed in Table 1 below.

 

Note: For special vaccination way, incubation time or training methods, please refer to instructions included with each biochemical identification tube.

 

 

Code	Name	Code	Name
075050	Sucrose biochemical identification tube	075370	Glucose gas
075040	Mannitol biochemical identification tube	075610	Acetamide
075030	Maltose biochemical identification tube	075600	Arginine double hydrolase control
075020	Lactose biochemical identification tube	075590	Arginine double hydrolase
075010	Glucose biochemical identification tube	075580	Arginine decarboxylase
075150	Urea biochemical identification tube	075570	Cellobiose
075130	Adonis Aging identification tube	075560	Melibiose
075140	Salicin biochemical identification tube	075550	Fructose
075120	Inositol biochemical identification tube	075540	Inulin
075110	Rhamnose biochemical identification tube	075530	Mushroom sugar
075100	Dulcitol biochemical identification tube	075522	Mucate QC broth
075090	Sorbitol biochemical identification tube	075720	Meilan milk 0.1%
075080	Galactose biochemical identification tube	075700	40% of the bile broth
075070	Xylose biochemical identification tube	075630	PH9.6 broth
075060	Arabinose biochemical tube	075680	6.5% high-salt broth
075260	Glucose ammonium biochemical identification tube	075670	UIM(Urea Indole Motility Medium)
075250	Glucose phosphate broth biochemical identification tube	075660	SIM ( Sulfide Indole Motility Medium)
075240	Peptone water broth biochemical identification tube	075650	Purple milk
075230	Raffinose biochemical identification tube	075640	OF (Hugh-leifson)
075220	Phenylalanine biochemical identification tube	075630	Nitrite (gas)
075210	Gluconate biochemical identification tube	075620	Nitrate (gas)
075200	Simon's citrate biochemical identification tube	075850	Lactose gelatin
075190	Malonate biochemical identification tube	075840	Glycerol fermentation tube
075180	B- galactosidase, ONPG biochemical identification tube	075810	Buffer power - nitrate
075160	Esculin biochemical identification tube	075800	Acetamide broth
075360	5% lactose	075780	Koser's citrate broth
075350	Semisolid agar	075770	Hippurate
075260	Potassium cyanide control tube (KCN) biochemical identification tube	075760	Glucose semisolid
075330	Potassium cyanide biochemical identification tube	075750	Triple sugar iron
075260	Nitrate (reducing) biochemical identification tube	075740	Bile lysis control
075310	Gelatin biochemical identification tube	075730	Bile bacteriolysis
075300	Hydrogen sulfide biochemical identification tube	075510	DNA
075290	Amino acid decarboxylase control biochemical identification tube	075490	Acetate
075280	Lysine decarboxylase biochemical identification tube	075480	Starch
075270	Ornithine decarboxylase biochemical identification tube	075440	Sorbitol
075521	Mucate test broth	075430	Melizitose
075420	Turanose	075400	D- ribose
075380	Mannose

Packaging & Shipping

Packing material: Packed in westwood vials with plastic bottom
Capacity: 20 pcs / unit

Our Services
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.

FAQ
Q1:Sometimes color of dehydrated culture media  between batches have subtle differences,would this affect test results?

A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.

 

Q2:After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?

A2: The reason may be:

The required hydration in the preparation process have not been done completely .That is to say   distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.

 

Q3:Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?

A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.

 

Q4:During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?

A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.

Company Information