| CAS No.: | 3 Years |
|---|---|
| Formula: | Culture Medium |
| EINECS: | Macconkey Agar |
| Classification: | Biochemical Reagents |
| Grade: | Br |
| Specific Usage: | For Biological Purpose, For Microbiological, Technical Grade, Pratical Use, Pro Analysis |
| Customization: |
|---|
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Audited Supplier HCM017 Macconkey Agar
Usages:
For isolating lactose-fermenting Gram-negative enteric bacilli.
Principle:
Peptones are sources of nitrogen and other nutrients. Lactose is a fermentable carbohydrate. When lactose is fermented, alocal pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts,bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Agar is the solidifying agent.
Formulation(per liter):
Pancreatic Digest of Gelatin 17.0 g
Peptones (meat and casein) 3.0 g
Lactose Monohydrate 10.0 g
Sodium Chloride 5.0 g
Bile Salts 1.5 g
Agar 13.5 g
Neutral Red 30.0 mg
Crystal Violet 1 mg
Final pH 7.1±0.2
How to use:
1.Suspend 50 g in 1 L of distilled or deionized water. Heat to boiling to dissolve completely. Autoclave at 121°C for 15 minutes.
2.transfer 1 mL of Soybean-Casein Digest Broth to 100 mL of MacConkey Broth, and incubate at 42to 44for 24 to 48 hours. Subculture on a plate of MacConkey Agar at 30to 35deg.C for 18 to 72 hours.
Quality control:
Item The name and number of strain PR/G Reaction
Growth rate E.Coli ATCC8739 PR≥0.7 Rose-red
Characteristic difference Proteus mirabilis CMCC(b)49005 PR≥0.7 Colorless,
no swarming
Selective Staphylococcus aureus ATCC6538 G≤1 -
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of three years.
Specifications: 500g/bottle
HKM Company has been in Microbial Detection since 1993,
with manufacture base over 20,000 sq.m ,1200 sq.m of Microbiological Research Lab and GMP room and 300 staffs,HKM has a full range of Culture Media as below :
Dehydrated Culture Media
Granular Media
Chromegenic Media
Ready-to-use Culture Media
Triple wrapped contact plate & settle plate
Media Raw Materia
Other Microbial Detection Products
Package:
500gr / bottle :Packed in plastic bottles with plastic film wrapped, 20 bottles / carton,
Carton size :54.5*34.5*21 CM
Other packing also available according to customers demand.
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Q1:
Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:
After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:
Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:
During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
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Audited Supplier 
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