| CAS No.: | Culture Medium |
|---|---|
| Formula: | Culture Media |
| EINECS: | Chromogenic Vibro Agar |
| Classification: | Biochemical Reagents |
| Grade: | Br |
| Specific Usage: | For Biological Purpose, For Microbiological, Technical Grade, Pratical Use |
| Customization: |
|---|
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Audited Supplier CRM008 Vibrio Chromogenic Medium
(Chromogenic Vibrio Agar)
Usages:
For isolation and identification of Vibrio especially for parahaemolyticus Vibrio in aquatic products and food poisoning samples.. (GB / T4789.7-2008, SN0173-2010 and SN1022-2010)
Principle:
Peptone and yeast extract powder to provide a nitrogen source, vitamins, amino acid as carbon source; sucrose fermentable sugars; most non-bacteriostatic agents inhibit Vibrio bacteria, sodium chloride can be maintained a balanced osmotic pressure; the agar medium coagulant; mixing pigment with Vibrio cholerae and Vibrio vulnificus corresponding enzyme parahaemolyticus specific reaction, hydrolysis of the substrate, the release of the color groups to produce magenta colonies (deputy in the pale yellow tablet Vibrio parahaemolyticus) and green - blue-green colonies (Vibrio cholerae and Vibrio vulnificus).
Formulation(per liter):
Peptone :18.8g
Yeast extract powder:5g
Sucrose: 20g
Sodium chloride:10g
Bacteriostatic agent :1.5g
Agar: 13g
Mixing pigment: 3g
Final pH 9.0 ± 0.2
How to use :
1, Take 71.3g of the powder, dissolved in 1000ml of distilled or purified water, according to the proportion of the amplified or reduced. Heated to boiling stirring until completely dissolved, without autoclaving, cooled to about 50 , pour sterile petri dish.
2, Take 25 g (mL) of test sample with aseptical procedure, add 3% sodium chloride alkaline peptone water 225 mL, with a rotating blade homogenizer at 8 000 r / min homogenized 1 min, or slap-style homogenizer slap 2 min, or pulsed with Pulsifier homogeneous sample processor for 30 seconds to prepare a uniform dilution 1:10. If no homogenizer, then samples were placed in a sterile mortar and ground, and then placed in 500 mL of sterile container, add 225 mL3% sodium chloride, alkaline peptone water and thoroughly shaken.
3, enrichment: 1:10 dilution above were cultured in 36 ± 1 8 ~ 18h.
4, the separation
Used in the enrichment broth inoculation loop take part, in Vibrio color separation crossed the plate and incubated at 36 ± 1 18 ~ 24 h.
5, further confirm presumptive Vibrio parahaemolyticus and Vibrio other validated tests, such as oxidase, Gram stain, biochemical identification.
Quality control:
This product appears light yellow after the pour plate, these strains were inoculated after 36 ± 1 cultured for 18-24 hours growth in the following table:
Bacteria name inoculum (cfu / plate) growth situation feature
Vibrio parahaemolyticus ATCC17802 30 - 300 good red
VBO Vibrio cholerae non-01 30 - 300 good green-blue
Alginolyticus ATCC33787 _____ good colorless
Escherichia coli ATCC25922 5000 no growth ___
Note: The additional test for final identification of need to be done.
Appearance:
Dehydrated Culture Media: light yellow powder with liquidity.
Good preparation tablet: yellow plastic.
Storage conditions and shelf life:
Dehydrated Culture Media: 10-30 , the shelf life of two years.
Prepared Medium plate: 2-8 , save up to two weeks.
References:
1. GB / T4789.7-2008 People's Republic of China national standards of food hygiene Microbiological examination of Vibrio parahaemolyticus test
2. SN0173-2010 People's Republic of China Entry-Exit Inspection and Quarantine export industry standards Vibrio parahaemolyticus in food inspection
3. SN1022-2010 People's Republic of China Entry-Exit Inspection and Quarantine industry standard export food inspection in Vibrio cholerae
1.Offering Free Sample for Testing .
2.R & D team to provide technical support.
3.Provide packaging, labeling OEM customization.
Q1:
Sometimes color of dehydrated culture media between batches have subtle differences,would this affect test results?
A1:The source and storage conditions of raw material is different ,so there may be a slight difference in color, which is a normal phenomenon. Products from our company have to undergo a rigorous inspection and sensory biology verification before sale, to ensure that all the indicators characteristics of the product is under enterprise standard to the extent permitted, not affect the test results.
Q2:
After pouring the liquid medium on the plate , medium seems hard to clot or coagulation time is longer, is it some problem with product quality?
A2: The reason may be:
The required hydration in the preparation process have not been done completely .That is to say distilled water is not boil sufficiently to dissolve agar. Because agar proportion, easily settle in the bottom of the bottle, if not fully shaken after high-temperature sterilization, it will lead to uneven upper agar culture gene content and difficult to set.
Q3:
Why some colonies of E. coli chromogenic medium color not shown above, but also confirmed through biochemical tests for E. coli (false negative)?
A3: E. CHROMOGENIC medium is based on the principle of specific enzyme of E. coli and design, 94% of E. coli have β- glucuronidase enzyme, with the chromogenic enzyme substrate medium role in the formation of blue-green colonies. And about 4% of the E. coli does not have β- glucuronidase enzymes, including concern O157: H7 Escherichia coli. Therefore, these E. coli can not be displayed on the color characteristic of E. coli chromogenic medium,it is possible of false-negative on the chromogenic medium. However, the traditional medium of the same can not avoid the problem of false negatives, such as: no gas or slow ferment lactose intolerance may also strain 44.5 false negatives in the conventional medium. For this small part of the E. coli can be detected using other methods.
Q4:
During anaerobic or micro-aerobic culture, is it necessary to remove gas bag from package? How to use oxygen indicator?
A4: During anaerobic or micro-aerobic culture, using method of gassing agent should be done according to the manufacturer's instructions. Remove the paper bag from the plastic packaging, but do not cut the paper bag. Torn the outer packaging of oxygen indicator then it can be used.
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